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Table 1 Antibody conditions for tissue staining

From: Macrophage to myofibroblast transition contributes to subretinal fibrosis secondary to neovascular age-related macular degeneration

Ab name Species Company Secondary antibody Company
Collagen-1 Rabbit Abcam: ab34710 594 Donkey anti-rabbit or 488 donkey anti-rabbit Jackson: 711-585-152
Jackson: 712-545-150
F4/80 Rat Bio-Rad: MCA497RT 594 Donkey anti-rat IgG or 488 donkey anti-rat Jackson: 712-585-150
Jackson: 712-545-150
α-SMA Mouse Sigma-Aldrich: C6198 -Cy3 conjugated
Iba-1 Rabbit Wako: 013-2769 488 Donkey anti-rabbit Jackson: 712-545-150
C3a Rat BD Biosciences: 558251 Goat anti-rat-594 Invitrogen: A11007
C3aR Chicken Peninsula Laboratories International: T-2301 Biotinylated anti-chicken followed by streptavidin 488 Jackson: 703-065-155
ThermoFisher: S11223
Biotinylated Isolectin-B4 VectorLabs: B-1205 Streptavidin-488 ThermoFisher: S11223
TGF-β Rabbit Abcam: ab92486 594 Donkey anti-rabbit Jackson: 711-585-152