Fig. 2.

Dynamic changes in protein expression of CCL3 and its receptors following SCI. a CCL3 protein in spinal cord from uninjured (laminectomy only) and 1, 7, 14, 28 dpi mice was quantified using ELISA, which revealed a significant increase at day 1 following injury. By day 7, expression decreased to basal levels and was elevated again at day 14 and 28. Values are shown as mean ± SEM, n = 5–7 per group, *<p value 0.05. b The receptor protein expression was assessed using western blot with normalization to GAPDH. CCR1 and CCR5 were significantly upregulated at day 7 and 28 post-injury. CCR4 was not changed at any time point compared to uninjured mice. Representative images of Western blots are shown. Values are shown as mean ± SEM, n = 4–7 per group, * < p value 0.05