Fig. 4

Elevated levels of reactive astrocytes after BCAS that are not exacerbated by deficiency of Nrf2. a There was a significant effect of genotype on GFAP immunostaining (% area) in the corpus callosum but there was no effect of BCAS surgery. b Percent area of GFAP was unchanged in the internal capsule. c There was a significant effect of BCAS surgery on percent area of GFAP immunostaining in the optic tract, but the effect of genotype narrowly missed accepted levels of statistical significance. Mean ± SEM. **p < 0.01, ***p < 0.001 (asterisk indicates post hoc differences between sham and BCAS), #p < 0.05 (number sign indicates post hoc differences between wild type and Nrf2^−/−). Group sizes presented in each bar. d Representative images of GFAP staining in the optic tract. Scale bar 50 μm