Fig. 2

RD-associated leukocyte infiltration is associated with cone loss. a Representative cytometry plots of mouse retina without RD (upper panels) or 7 days after RD (lower panels). CD45⁺CD3⁺ cells define T cells and CD45⁺CD11b⁺ cells define microglia and myeloid cells with CD45^lowCD11b⁺ representing microglia and CD45^highCD11b⁺ representing infiltrating myeloid cells. CD45^highCD11b⁺ were further defined with Ly6C and Ly6G with Ly6C^highLy6G^- cells representing monocytes, Ly6C^lowLy6G^- representing macrophages and Ly6G⁺ representing granulocytes. Flow cytometry quantification of b T cells, MCs, and infiltrating myeloid cells and c Mos, Mφs, and neutrophils in mouse retina without RD and 24 h, 3 days, and 7 days after RD (n = 8–16/group; b one-way Anova *p < 0.006, **p < 0.0001, and ^$p < 0.05 versus the control group; c one-way Anova *p = 0.0016, **p < 0.0001, and ^$p = 0.0384 versus the control group). d Representative images of the subretinal aspect of retinal flatmounts of peanut agglutinine- (PNA, red), cone arrestin- (CAR, green), and ionized calcium-binding adapter molecule 1- (IBA-1, white) stained retina of C57BL6/J mice without RD, 24 h, 3 days, and 7 days after RD. Quantification of e subretinal IBA-1+ cells, f PNA+ cones, and g CAR+ cones in retinal flatmounts of C57BL6/J mice without RD and 1 day, 3 days, 7 days, and 10 days after RD (n = 6–12/group; e one-way Anova *p = 0.0153 and ^$p < 0.0001 versus the control group; f one-way Anova *p < 0.0001 versus the control group; g one-way Anova *p < 0.0001 versus the control group). RD, retinal detachment; Myeloid, myeloid cells; cMos, classical monocytes; MCs, microglial cells; ncMos/Mφs, non-classical monocytes/macrophages; Neutros, neutrophils; PNA, peanut agglutinine; CAR, cone arrestin; IBA-1, ionized calcium-binding adapter molecule 1. Scale bar (d) = 50 μm. All values are reported as mean ± SEM