Fig. 3

Microglia alteration in the SC of HSV-2^esi latently infected mice. a WBs for Iba1 (Mock and HSV-2^esi (n = 7 from two independent experiments)) and P2ry12 (Mock and HSV-2^esi (n = 8 from two independent experiments)). ***p < 0.001, **p < 0.01. Each lane corresponds to one SC. b Z-stack confocal images of Mock (n = 4) and HSV-2^esi (n = 5) SCs from two independent experiments. CD45 in green, Iba1 in red, NeuN in white, and Dapi in blue. Open red arrowheads point to microglia in the white matter; solid red arrowheads point to microglia in the gray matter; open yellow arrowheads point to macrophages (MΦ) in the white matter; solid red arrowheads point to MΦ in the gray matter. c Single-plane confocal images of Mock (n = 4) and HSV-2^esi (n = 5) SCs from two independent experiments. CD45 in green, Iba1 in red, NeuN in white, and Dapi in blue. Open red arrowheads point to microglia in the white matter; solid red arrowheads point to microglia in the gray matter; open yellow arrowheads point to MΦ in the white matter; and solid yellow arrowheads point to MΦ in the gray matter. d Quantification of CD45^high Iba1⁺ versus CD45^low Iba1⁺ cells in the white matter (left graph) and in the gray matter (right graph). n = 5 from two independent experiments. e Z-stack confocal images of Mock and HSV-2^esi SCs. CD45 in green, Iba1 in red. f Quantification of the number of ramified CD45^low Iba1⁺ cells in the gray matter. Mock (n = 4) and HSV-2^esi (n = 5) from two independent experiments (left graph). Quantification of the area of ramified CD45^low Iba1⁺ cells in the gray matter. Mock (n > 2000) and HSV-2^esi(n > 3000) from two independent experiments (right graph). *p < 0.05, ***p < 0.001