Fig. 6

C9orf72 decreased levels in the SC of HSV-2^esi latently infected mice. a WBs for C9orf72 (Mock and HSV-2^esi (n = 8 from two independent experiments)) and SOD1 (Mock and HSV-2^esi (n = 7 from two independent experiments, p = 0.248)). Open arrowheads point to 55 kDa and 50 kDa C9orf72 isoforms. ***p < 0.001. Each lane corresponds to one SC. b Z-stack confocal images of Mock (n = 4) and HSV-2^esi (n = 5) SCs from two independent experiments. C9orf72 in red, NeuN in white, and Dapi in blue. c Single-plane confocal images of HSV-2^esiSCs (n = 5) from two independent experiments. C9orf72 in red, MHC-II in green, NeuN in white, and Dapi in blue. Open yellow arrowhead points to a C9orf72⁺MHC-II^high cell in the white matter. d Z-stack confocal images of Mock (n = 3) and HSV-2^esi (n = 3) SCs. Lamp1 in green, Iba1 in red. Quantification of the area of Lamp1 in ramified Iba1⁺ cells in the gray matter. Mock and HSV-2^esi (n > 100) from three independent mice. ***p < 0.001