Fig. 2

Developmental expression of mGlu3 receptors following the LPD/IL-1β double hit in rat. a, b Grm3 mRNA expression in cortex (a) and in microglia (b) sorted from CTRL and LPD/IL-1β animals. Data (mean ± SEM) are relative to Grm3 expression in sorted cells of CTRL cortex and CTRL microglia at PND1. Two-way ANOVA followed by the Newman-Keuls multiple comparison test; **p < 0.01, ****p < 0.0001 vs respective P1; ^&&p < 0.01, ^&&&&p < 0.0001 vs respective P4; ^$$p < 0.01, ^$$$p < 0.001 vs respective P7; ^xxxp < 0.001 vs respective P10; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 effect of LPD/IL-1β. c–f mGlu3 protein quantification in cortex and primary microglia cells at P4 (c, e) and P7 (d, f) in CTRL and LPD/IL-1β pups. P4 cortex and microglia cells sorted from three animals were pooled. Data (mean ± SEM). Quantification of mGlu3 receptor expression in tissue or cells from LPD/IL-1β pups was performed relative to that of CTRL pups. The optical density (O.D.) for mGlu3 receptors was normalized to the O.D. of Ponceau S staining. Unpaired t test; ^#p < 0.05, ^##p < 0.01