Fig 3.

Neutrophil infiltration of the spinal cord lesion peaks 24 hours post-injury. Female C57BL/6 mice underwent either a 70kD SCI (black bars, n=4-5/time point) or sham surgery (white bars, n=5/time point). Tissue was collected at 2, 6, 12 and 24 hours post-operation and was compared to naïve baseline (grey bar and dotted line, n=5). Neutrophil cell density was calculated at the lesion epicentre (a). Representative images of neutrophil density in the epicentre of spinal cord lesions at each time point (b). Scale bar represents 50μm. Data are presented as mean ±SEM. Two-way ANOVA, ****p<0.0001 with post hoc test