Fig 4.
IL-1RA treatment significantly attenuates peripheral inflammation 6 hours post-injury. Female C57BL/6 mice underwent a 70kD SCI and were treated with human IL-1RA (i.p. 100mg/kg, n=8) immediately following, and 5 hours after impact, or saline as control (n=9). Tissue was collected 6 hours post-injury and compared to naïve baseline (dotted line, n=5). Relative expression of IL-1β (a), CXCL10 (b), CXCL1 (c) and SAA-2 (d) in the liver, were measured by qPCR. Neutrophil infiltration of the liver was determined by immunohistochemistry (ei). Representative images of neutrophils staining in the liver (eii). Scale bar represents 50μm. Data are presented as mean ±SEM and were analysed by student’s unpaired t-test, *p<0.05, **p<0.01