Fig. 7

Effect of Nostrill induction on microglial-mediated neurotoxicity. a Illustration of co-culture experiments. Briefly, brains were dissected, cortices were dissociated, and cortical cells were cultured for 1 week. BV2s were plated on transwells, transfected, and stimulated with LPS (10 μg/ml) for 6 h. Media was then exchanged for fresh Neurobasal media, and the transwells containing stimulated BV2s were transferred to co-culture with the cortical cells for 3 days. Cortical cells were subsequently fixed and stained. Illustration created using BioRender. b Impact of Nostrill knockdown on microglial-mediated neurotoxicity. Cortical cells were stained with β-tubulin III and DAPI. Scale bar = 50 μm. Relative fluorescent units were quantified in (c). ***p < 0.001 vs siRNA-control. †p < 0.05 between indicated groups. d Impact of Nostrill overexpression on microglial-mediated neurotoxicity. Cortical cells were stained with neuron-specific, β-tubulin III, and DAPI. Scale bar = 50 μm. Relative fluorescent units were quantified in (e). ###p < 0.001 vs empty vector. Data represent means ± SEM from three independent experiments