Fig. 2

Cellular response following exposure to pure Hb-metabolites. Representative images are from mixed glial cell cultures following exposure to the Hb-metabolites oxyHb (2.5 mg/ml), oxidized Hb (2.5 mg/ml), and heme (15 μM). Images illustrate the detected IF labeling of Iba1 (red; b, d, f, h, j, l, n, p), F-actin cytoskeletal protein (phalloidin, green; c, d, g, h, k, l, o, p) and DAPI nuclear staining (blue; a, d, e, h, i, l, m, p), in the wells of mixed glial cells exposed to culture media only (control; a–d) and in wells exposed to pure Hb-metabolites (oxyHb 2.5 mg/ml, e–h; oxidized Hb 2.5 mg/ml, i–l; and heme 15 μM, m–p). d, h, l, p Merged images of DAPI, Iba1, and phalloidin. Scale bar in p indicates 200 μm and is representative for a–p