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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Cell-free oxidized hemoglobin drives reactive oxygen species production and pro-inflammation in an immature primary rat mixed glial cell culture

Fig. 2

Cellular response following exposure to pure Hb-metabolites. Representative images are from mixed glial cell cultures following exposure to the Hb-metabolites oxyHb (2.5 mg/ml), oxidized Hb (2.5 mg/ml), and heme (15 μM). Images illustrate the detected IF labeling of Iba1 (red; b, d, f, h, j, l, n, p), F-actin cytoskeletal protein (phalloidin, green; c, d, g, h, k, l, o, p) and DAPI nuclear staining (blue; a, d, e, h, i, l, m, p), in the wells of mixed glial cells exposed to culture media only (control; ad) and in wells exposed to pure Hb-metabolites (oxyHb 2.5 mg/ml, eh; oxidized Hb 2.5 mg/ml, il; and heme 15 μM, mp). d, h, l, p Merged images of DAPI, Iba1, and phalloidin. Scale bar in p indicates 200 μm and is representative for ap

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