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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Cell-free oxidized hemoglobin drives reactive oxygen species production and pro-inflammation in an immature primary rat mixed glial cell culture

Fig. 4

Dose-dependent effect of oxidized Hb. Mixed glial cells were exposed to increasing concentration of oxidized Hb (5 mg/ml, dark grey bars, n = 6; 10 mg/ml, light grey bars, n = 6) or culture medium only (control, white bar, n = 6) for 24 h. Subsequently, ROS production (a), mRNA expression of iNOS (b) and protein secretion of CCL2 (c) and CCL5 (d) were analyzed. Results are presented as box plots displaying medians and 25th and 75th percentiles. Differences between 10 mg/ml oxidized Hb vs. control, 5 mg/ml oxidized Hb vs. control, and 10 mg/ml oxidized Hb vs 5 mg/ml oxidized Hb were analyzed using the Kruskal–Wallis test followed by pairwise comparison with significance values adjusted for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001

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