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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Cell-free oxidized hemoglobin drives reactive oxygen species production and pro-inflammation in an immature primary rat mixed glial cell culture

Fig. 5

Effect of Hp on the damage induced by oxidized Hb. Mixed glial cells were exposed to oxidized Hb (10 mg/ml, dark grey bars, n = 6) with or without co-administration of Hp2-2/2-1 (40 mg/ml, corresponding to a 1:1 molar ratio, light grey bars, n = 6) or culture medium only (control, white bars, n = 6) for 24 h. Subsequently, ROS production (a), mRNA expression of HO-1 (b), SOD2 (c), and iNOS (d) were analyzed. Results are presented as box plots displaying medians and 25th and 75th percentiles. Differences between oxidized Hb vs. control and oxidized Hb with co-administered Hp vs. control were analyzed using the Kruskal–Wallis test followed by pairwise comparison with significance values adjusted for multiple comparisons. **P < 0.01, ***P < 0.001

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