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Table 1 Hb-binding activity of Hp2-2/2-1. Equilibrium constant, association and dissociation constants for the biolayer interferometry experiments. Data expressed as mean ± SD from three independent experiments

From: Cell-free oxidized hemoglobin drives reactive oxygen species production and pro-inflammation in an immature primary rat mixed glial cell culture

Hb-metabolite

KD (pM)

kon (1/Ms)

kdis (1/s)

OxyHb

128.7 ± 2.0

2.7 × 105

3.5 × 10−5

MetHb

141.4 ± 1.8

2.0 × 105

2.8 × 10−5