Fig. 1

Microglia immunofluorescence of different brain areas in APP/PS1 and WT mice. a Immunofluorescence demonstrated that the Iba-1⁺ area in the b medial prefrontal cortex (PFC), c anterior cingulate cortex, and d hippocampal DG, and e CA3 increased significantly with aging in WT and APP/PS1 mice. In 12-month-old APP/PS1 mice, Iba-1 expression was significantly more than that of 12-month-old WT mice and 3-month-old APP/PS1 mice. In addition, in PFC, ACC, and DG, the Iba-1⁺ area in 12-month-old APP/PS1 mice was significantly more than in 24-month old WT mice. n = 6. ns, not significant. **P < 0.01. ****P < 0.0001. #P < 0.0001 compared with 12-month-old APP/PS1 mice. ▲P < 0.05 compared with 12-month-old WT mice. ★P < 0.0001 compared with 12-month-old APP/PS1 mice. ΔP < 0.05 compared with 24-month-old WT mice. ΔΔΔP < 0.001 compared with 24-month-old WT mice. ΔΔΔΔP < 0.0001 compared with 24-month-old WT mice