Fig. 8

Detection of NET⁺ nerve fibers in APP/PS1 and WT mice. a Immunofluorescence detected NET⁺ nerve fibers in b medial prefrontal cortex, c anterior cingulate cortex, d DG, and e CA3. In these areas, NET⁺ nerve fibers gradually decreased with aging. NET⁺ nerve fibers in 12-month-old APP/PS1 mice were significantly less compared with WT mice of the same age and 3-month-old APP/PS1 mice. In ACC, DG, and CA3 of 12-month-old APP/PS1 mice, the NET⁺ area was less than that of 24-month-old WT mice. n = 6. Comparisons between APP/PS1 mice and WT mice and comparisons among 3 WT groups were estimated with two-way ANOVA and followed by Tukey’s post hoc multiple comparison tests. ns, not significant. **P < 0.01, ***P < 0.001, ****P < 0.0001, #P < 0.05 compared with other time points in the same group. ▲P < 0.05 compared with 12-month-old WT mice. ★P < 0.01 compared with 12-month-old APP/PS1 mice