Fig. 6

The microglial P2X7R increased in the TNC after chronic administration of NTG. a Representative western blot of P2X7R and CGRP on different time points in the TNC. b, c The band intensities of P2X7R and CGRP relative to those of β-actin indicated that the protein level of P2X7R (b) and CGRP (c) increased gradually with the development of CM model. n = 6 per group; data are presented as mean ± SEM; one-way ANOVA and Dunnett’s multiple comparison test; **p < 0.01 and ***p < 0.001 compared with the SHAM group. d Double immunofluorescence staining of P2X7R and Iba1, GFAP, and NEUN in the TNC area. Most P2X7R-positive cells are double labeled with Iba1. Scale bar: 20 μm