Table 1 Antibody used in western blotting analysis, immunofluorescence staining assays
Antibody | Manufacturer | Catalog number | Host | Dilution |
|---|---|---|---|---|
For western blot analysis | ||||
 P2X7 | Sigma-Aldrich, USA | P8232 | Rabbit | 1:3000 |
 LC3 | Bimake, USA | A5202 | Rabbit | 1:500 |
 Beclin1 | Cell signaling technology, USA | 3495 | Rabbit | 1:1000 |
 P62 | Cell signaling technology, USA | 5114T | Rabbit | 1:1000 |
 NLRP3 | Abcam, UK | ab205680 | Rat | 1:1000 |
 IL-1β | Bioss, China | bs-6319R | Rabbit | 1:1000 |
 IL-18 | Abcam, UK | ab207323 | Rabbit | 1:1000 |
 CGRP | Abcam, UK | Ab139264 | Rabbit | 1:3000 |
 c-fos | Santa Cruz, USA | sc-447 | Mouse | 1:1500 |
 β-actin | ZSGB-BIO, China | TA-09 | Mouse | 1:3000 |
 HRPa conjugated anti-rabbit | ZSGB-BIO, China | ZB-2301 | Goat | 1:9000 |
 HRP conjugated anti-mouse | ZSGB-BIO, China | ZB-2305 | Goat | 1:5000 |
 HRP conjugated anti-rat | ZSGB-BIO, China | ZB-2307 | Goat | 1:5000 |
For immunofluorescence staining | ||||
 P2X7 | Sigma-Aldrich, USA | P8232 | Rabbit | 1:500 |
 CGRP | Santa Cruz, USA | sc-57053 | Mouse | 1:100 |
 c-fos | Novus Biologicals, USA | NBP2-50057SS | Rabbit | 1:5000 |
 Iba1 | Wako, Japan | 019-19741 | Rabbit | 1:800 |
 Iba1 | Novus Biologicals, USA | NB100-1028SS | Goat | 1:800 |
 GFAP | Santa Cruz, USA | sc-33673 | Mouse | 1:200 |
 NeuN | Abcam, UK | ab104224 | Mouse | 1:500 |
 Cy3 Goat anti-rabbit IgG | Beyotime, China | A0516 | Goat | 1:500 |
 Alexa Fluor 488 Goat anti-mouse IgG | Beyotime, China | A0428 | Goat | 1:500 |
 Alexa Fluor 555 Donkey anti-rabbit IgG | Beyotime, China | A0453 | Donkey | 1:500 |
 IFkine Green, Donkey anti-goat IgG | Abbkine, USA | A-24231-1 | Donkey | 1:400 |