Fig. 2

The effects of L-NAME on the amounts of SNO-thiol- and total thiol-P2X7R following SE. As compared to vehicle (V), L-NAME (L) reduces the amount of SNO-thiol-P2X7R and increased that of total thiol-P2X7R without altering P2X7R expression under physiological condition. Thus, S-nitrosylation ratio of P2X7R is decreased. SE upregulates P2X7R expression and S-nitrosylation ratio of P2X7R due to the reduced the amount of total thiol without the altered that of SNO-thiol-P2X7R. L-NAME inhibits these alterations induced by SE, except P2X7R expression. a Representative Western blot for S-nitrosylation and thiolization on P2X7R and its expression. b-e Quantification of analyses of S-nitrosylation and thiolization on P2X7R and its expression. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM (*^#p < 0.05 vs. control animals and vehicle, respectively; n = 7, respectively). f Representative photos of the localization of P2X7R in the CA1 region following SE. Upregulation of P2X7R expression induced by SE is restricted to microglia