Fig. 4

The effects of L-NAME on astroglial death and reactive astrogliosis in the hippocampus following SE. SE leads to astroglial apoptosis in the molecular layer of the dentate gyrus and reactive astrogliosis in the CA1 region, respectively. L-NAME ameliorates SE-induced astroglial apoptosis, but not reactive astrogliosis. a Representative photos for TUNEL-positive astrocytes in the dentate gyrus following SE. b Quantification of analyses of the effect of L-NAME on GFAP-positive area following SE. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM (*^#p < 0.05 vs. control animals and vehicle, respectively; n = 7, respectively). c Representative photos for double immunostaining for TUNEL and GFAP in the CA1 region following SE. Reactive CA1 astrocytes do not show TUNEL signals following SE