Fig. 11
From: Propofol suppresses microglial phagocytosis through the downregulation of MFG-E8
Effects of AMPK and Src pathway modulation on MFG-E8-mediated microglial phagocytosis. BV2 cells were treated with dasatinib (100 nM, 30 min) and compound C (10 μM, 1 h), treated with propofol, and then incubated with MFG-E8 (100 ng/ml) and fluorescence-labeled latex beads. Flow cytometry (a, b) and immunofluorescence (c) were used to assess the microglial phagocytosis of latex beads. Immunofluorescence staining: phalloidin-red, latex beads-green, DAPI-blue. The data are presented as the mean ± SD. Pro, propofol; M8, MFG-E8; CC, compound C; DAS, dasatinib. *P < 0.05 versus the control; #P < 0.05 versus propofol; ^P < 0.05 versus propofol+MFG-E8