Fig. 5
From: Propofol suppresses microglial phagocytosis through the downregulation of MFG-E8
Blockage of MFG-E8 on the phagocytosis ability of microglia by propofol. BV2 cells were treated with or without an MFG-E8 antibody (5 μg/ml)/IgG antibody (5 μg/ml) for 45 min after treatment with propofol (12.5 μM) for 4 h. Flow cytometry (a, b) and immunofluorescence (c) were used to assess the microglial phagocytosis of latex beads. Immunofluorescence staining: phalloidin-red, latex beads-green, DAPI-blue. The data are presented as the mean ± SD. Pro, propofol; M8 Ab, MFG-E8-neutralizing antibody. *P < 0.05 versus the control