Fig. 1
From: Chronic exposure to IL-6 induces a desensitized phenotype of the microglia
Microglial cell activation. Representative images from WT and GFAP-IL6Tg mice showing Iba1 staining in the granular (GL) as well as the inner, medial, and outer molecular layer (IML, MML, and OML, respectively) of the dentate gyrus (DG) in non-lesioned (NL) and PPT-lesioned hippocampus from 3 to 21dpl. Scale bar = 20 μm (a) and 10 μm (f). b Graph showing the time course of Iba1 immunostaining, expressed as AI (area x intensity). In comparison with WT, GFAP-IL6Tg animals showed increased levels of Iba1 at 2 and 14dpl. c, d Graphs showing the fold change increase of Iba1 expression in comparison with the corresponding NL animals in both WT and GFAP-IL6Tg mice. e Graph showing the area occupied by Iba1 in the IML in both WT and GFAP-IL6Tg at 3 and 7 dpl. A minimum of 3 animals per genotype and experimental time-point were analyzed. A total of 9 photographs from 3 different hippocampal sections were used. Data are represented as mean ± SD. The significances are represented as #p ≤ 0.05 vs. NL of respective group; $p ≤ 0.05 vs. 7dpl of respective group. Significant differences between genotypes are represented as *p ≤ 0.05