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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Chronic exposure to IL-6 induces a desensitized phenotype of the microglia

Fig. 4

T cell infiltration after PPT. a Representative dot plot of SSC/CD3 expression of cells obtained from the lesioned WT animals. The square delimits the CD3+ cell population used in this study. Representative dot plot of CD3/CD4/CD8 cells obtained from the spleen, positive control (top), and hippocampus (bottom) of lesioned WT. b Graphs showing the number of CD4+, CD3+/CD4+/CD8+ cells, CD8+ and CD3+/CD4-/CD8 in NL and at 7dpl. c Representative histogram-plot showing the expression of Tbet, Gata3, RORgt, and Foxp3 in the population of CD3+Th-cells in comparison to the corresponding isotype control, in which the positive staining was defined. d Graphs showing the number of the different subtypes of CD4+ T-helper lymphocytes: Tbet+, Gata 3+, RORg+, and Foxp3+ in NL and at 7dpl. e Graph showing the quantification of CD3+ immunostaining cells in non-lesioned (NL) and PPT-lesioned hippocampus from 2 to 14 dpl, in WT and GFAP-IL6Tg mice. f Representative images showing CD3 immunostaining in WT and GFAP-IL6Tg mice at 7 dpl. Scale bar = 20 μm. A minimum of four WT and four GFAP-IL6Tg animals per group were used for this study. Data are represented as mean ± SD. The significances are represented as #p ≤ 0.05 vs. NL of respective group; $p ≤ 0.1 vs. NL of respective group and %p ≤ 0.05 vs. 3dpl of respective group. Significant differences between genotypes are represented as *p ≤ 0.05

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