From: Chronic exposure to IL-6 induces a desensitized phenotype of the microglia
 | Target antigen | Host | Dilution | Cat Number | Manufacturer |
---|---|---|---|---|---|
Primary antibodies | Iba1 | Rabbit | 1:3000 | 019-19741 | Wako |
Pu.1 | Rabbit | 1:400 | 2258S | Cell Signaling | |
CD45 | Rat | 1:1000 | MCA1031G | AbD Serotec | |
pH3 | Rabbit | 1:3000 | 06-570 | Millipore | |
BrdU | Rat | 1:120 | Ab6326 | Abcam | |
Caspase 3 | Rabbit | 1:1000 | AF835 | R&D Systems | |
Iba1 | Rabbit | 1:1000 | GTX100042 | Genetex | |
CD3 | Hamster | 1:500 | MCA2690 | AbD Serotec | |
MHCII (IA) | Rat hybridoma | 1:25 | TIB-120 | ATCC | |
CD206 | Rat | 1:500 | MCA2235GA | AbD Serotec | |
CD28 | Rabbit | 1:50 | ab203084 | Abcam | |
CTLA-4 | Rabbit | 1:25 | ab237712 | Abcam | |
Laminin | Rabbit | 1:500 | AMP420 | Biorad | |
TMEM119 | Rabbit | 1:1000 | ab209064 | Abcam | |
GFAP | Mouse | 1:3000 | 63893 | Sigma | |
Secondary antibodies | Biotinylated | Rabbit | 1:500 | BA-1000 | Vector Laboratories |
Biotinylated | Hamster | 1:500 | BA-9100 | Vector Laboratories | |
Biotinylated | Rat | 1:500 | BA-4001 | Vector Laboratories | |
Alexa 555 | Rat | 1:1000 | A11006 | Invitrogen | |
Alexa 488 | Rabbit | 1:500 | A21206 | Invitrogen | |
Cy2 | Mouse | 1:500 | PA42003 | GE Healthcare Lifescience | |
Streptavidin Alexa Fluor-555 | Â | Â | 1:500 | S32355 | Molecular Probes |
Streptavidin Alexa Fluor-488 | Â | Â | 1:500 | S11223 | Molecular Probes |
Streptavidin-HRP | Â | Â | 1:500 | SA-5004 | Vector Laboratories |
DAPI | Â | Â | 1:10000 | D9542 | Sigma Aldrich |