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Table 2 Reagents used for IHC

From: Chronic exposure to IL-6 induces a desensitized phenotype of the microglia

  Target antigen Host Dilution Cat Number Manufacturer
Primary antibodies Iba1 Rabbit 1:3000 019-19741 Wako
Pu.1 Rabbit 1:400 2258S Cell Signaling
CD45 Rat 1:1000 MCA1031G AbD Serotec
pH3 Rabbit 1:3000 06-570 Millipore
BrdU Rat 1:120 Ab6326 Abcam
Caspase 3 Rabbit 1:1000 AF835 R&D Systems
Iba1 Rabbit 1:1000 GTX100042 Genetex
CD3 Hamster 1:500 MCA2690 AbD Serotec
MHCII (IA) Rat hybridoma 1:25 TIB-120 ATCC
CD206 Rat 1:500 MCA2235GA AbD Serotec
CD28 Rabbit 1:50 ab203084 Abcam
CTLA-4 Rabbit 1:25 ab237712 Abcam
Laminin Rabbit 1:500 AMP420 Biorad
TMEM119 Rabbit 1:1000 ab209064 Abcam
GFAP Mouse 1:3000 63893 Sigma
Secondary antibodies Biotinylated Rabbit 1:500 BA-1000 Vector Laboratories
Biotinylated Hamster 1:500 BA-9100 Vector Laboratories
Biotinylated Rat 1:500 BA-4001 Vector Laboratories
Alexa 555 Rat 1:1000 A11006 Invitrogen
Alexa 488 Rabbit 1:500 A21206 Invitrogen
Cy2 Mouse 1:500 PA42003 GE Healthcare Lifescience
Streptavidin Alexa Fluor-555    1:500 S32355 Molecular Probes
Streptavidin Alexa Fluor-488    1:500 S11223 Molecular Probes
Streptavidin-HRP    1:500 SA-5004 Vector Laboratories
DAPI    1:10000 D9542 Sigma Aldrich