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Table 2 Reagents used for IHC

From: Chronic exposure to IL-6 induces a desensitized phenotype of the microglia

 

Target antigen

Host

Dilution

Cat Number

Manufacturer

Primary antibodies

Iba1

Rabbit

1:3000

019-19741

Wako

Pu.1

Rabbit

1:400

2258S

Cell Signaling

CD45

Rat

1:1000

MCA1031G

AbD Serotec

pH3

Rabbit

1:3000

06-570

Millipore

BrdU

Rat

1:120

Ab6326

Abcam

Caspase 3

Rabbit

1:1000

AF835

R&D Systems

Iba1

Rabbit

1:1000

GTX100042

Genetex

CD3

Hamster

1:500

MCA2690

AbD Serotec

MHCII (IA)

Rat hybridoma

1:25

TIB-120

ATCC

CD206

Rat

1:500

MCA2235GA

AbD Serotec

CD28

Rabbit

1:50

ab203084

Abcam

CTLA-4

Rabbit

1:25

ab237712

Abcam

Laminin

Rabbit

1:500

AMP420

Biorad

TMEM119

Rabbit

1:1000

ab209064

Abcam

GFAP

Mouse

1:3000

63893

Sigma

Secondary antibodies

Biotinylated

Rabbit

1:500

BA-1000

Vector Laboratories

Biotinylated

Hamster

1:500

BA-9100

Vector Laboratories

Biotinylated

Rat

1:500

BA-4001

Vector Laboratories

Alexa 555

Rat

1:1000

A11006

Invitrogen

Alexa 488

Rabbit

1:500

A21206

Invitrogen

Cy2

Mouse

1:500

PA42003

GE Healthcare Lifescience

Streptavidin Alexa Fluor-555

  

1:500

S32355

Molecular Probes

Streptavidin Alexa Fluor-488

  

1:500

S11223

Molecular Probes

Streptavidin-HRP

  

1:500

SA-5004

Vector Laboratories

DAPI

  

1:10000

D9542

Sigma Aldrich