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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: miR-142 downregulation alleviates rat PTSD-like behaviors, reduces the level of inflammatory cytokine expression and apoptosis in hippocampus, and upregulates the expression of fragile X mental retardation protein

Fig. 8

FMRP is a target gene of miR-142 verified in vitro. a Schematic illustration indicating the conserved seed match of FMRP 3′UTR to miR-142-5p. The putative binding sites were predicted by TargetScan. b Relative luciferase activity expressed with firefly/Renilla luciferase activity. Luciferase was fused to the 3′UTR of FMRP containing wild-type or a mutated seed sites in the presence of miR-control or miR-142-5p. c The expression of FMRP in neuron-like cells by western blot analysis and the relative band intensity of FMRP protein in the miR-NC and miR groups. d The expression of NF-κB p65 in the nuclei of neuron-like cells by western blot analysis and the relative band intensity of NF-κB p65 protein in the control, FMR1, and sh-FMR1 groups. Bar graphs represent the mean ± SEM of independent experimental triplicates. *P < 0.05, **P < 0.01, ***P < 0.001

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Journal of Neuroinflammation

ISSN: 1742-2094