Fig. 3

Endometriosis upregulated neuronal TLR4. a Western blot analyzed the time course of TLR4 expression in the DRG from naive, sham, 3 days, 7 days, 14 days, and 21 days after surgery. b n = 4, one-way ANOVA following Bonferroni's post-hoc test, *p < 0.05, versus naive and sham. c–h Immunofluorescence showed neuronal distribution of TLR4 in rat DRGs from sham and POD14 rats. Scale bar 50 μm. i TLR4 was expressed in the large-, medium-, and small-sized neurons in the dorsal root ganglion. Immunofluorescence staining showed a significantly elevated percentage of TLR4-immunopositive cells in small-sized DRG neurons of POD14 rats compared with that in sham rats. n > 100 neurons from 3 rats in each group, *p < 0.05, EM 14d versus sham, chi-square test