Fig. 9

Attenuated mechanical pain by administration of LRU and MIP for EM rats. a, b Pre-intrathecal injection of LRU and MIP inhibited the development of EM-induced mechanical allodynia. Each administration was indicated by an arrow on the 3, 2, and 1 day before EM surgery. c, d Mechanical allodynia was alleviated by intrathecal injection of LRU and MIP in the early phase of EM operation. Each administration is indicated by an arrow on the 3, 4, and 5 days after EM surgery. LRU (20 μg) was administrated i.t. in a volume of 20 μl in endotoxin-free water (Veh). MIP (500 mM) was administrated i.t.in a volume of 20 μl. The control peptide was used in the control (Cont) group. N = 7 in each group, two-way ANOVA followed by Bonferroni’s post hoc test, *p < 0.05 EM + Veh versus sham + Veh and EM + LRU versus sham + LRU (for a and c); EM + Cont versus sham + Cont and EM + MIP versus sham + MIP (for b and d). ^#p < 0.05, EM + LRU versus EM + Veh (for a and c) and EM + MIP versus EM + Cont (for b and d)