Antibody | Host | Company | Catalog number | Dilution | Incubation conditions |
---|---|---|---|---|---|
HMGB1 | Rabbit | Cell signaling technology | #6893 | 1:1000 | Overnight 4 °C |
MyD88 | Rabbit | Abcam | ab2064 | 1:500 | Overnight 4 °C |
TLR4 | Rabbit | Invitrogen | 48-2300 | 1:1000 | Overnight 4 °C |
pNF-κB-p65 | Mouse | Cell signaling technology | #13346 | 1:1000 | Overnight 4 °C |
β-actin | Mouse | Cell signaling technology | #3700 | 1:2000 | Overnight 4 °C |
Anti-rabbit IgG horseradish peroxidase (HRP) | Goat | ZSGB-BIO | ZDR-5306 | 1:3000 | 1 h RT |
Anti-mouse IgG horseradish peroxidase (HRP) | Goat | ZSGB-BIO | ZDR-5307 | 1:3000 | 1 h RT |