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Table 1 List of primary and secondary antibodies used for western blotting analysis

From: Suppression of TLR4-MyD88 signaling pathway attenuated chronic mechanical pain in a rat model of endometriosis

Antibody

Host

Company

Catalog number

Dilution

Incubation conditions

HMGB1

Rabbit

Cell signaling technology

#6893

1:1000

Overnight 4 °C

MyD88

Rabbit

Abcam

ab2064

1:500

Overnight 4 °C

TLR4

Rabbit

Invitrogen

48-2300

1:1000

Overnight 4 °C

pNF-κB-p65

Mouse

Cell signaling technology

#13346

1:1000

Overnight 4 °C

β-actin

Mouse

Cell signaling technology

#3700

1:2000

Overnight 4 °C

Anti-rabbit IgG horseradish peroxidase (HRP)

Goat

ZSGB-BIO

ZDR-5306

1:3000

1 h RT

Anti-mouse IgG horseradish peroxidase (HRP)

Goat

ZSGB-BIO

ZDR-5307

1:3000

1 h RT