Fig. 7

Anxiety- and repetitive-like behavior is increased following intestinal inflammation in Sham-injured and TBI mice. Representative heatmaps of mouse presence in the light chamber during light-dark box testing carried out in the third DSS recovery week a. TBI mice spent less time in the light chamber compared to Naïve and Sham mice. This was further decreased with DSS administration (TBI + DSS). DSS administration also decreased time spent in the light chamber in Sham+DSS mice compared to Sham mice b. TBI and TBI + DSS mice had fewer entries into the light chamber compared to Naïve/Sham and Naïve+DSS mice, respectively. Light chamber entries were also decreased in Sham+DSS mice compared to Sham mice c. TBI and TBI + DSS mice exhibited increased latency to re-enter the light chamber from the dark chamber compared to Naïve/Sham and Naïve+DSS/Sham+DSS mice, respectively. DSS administration significantly increased light chamber re-entry latency in Sham+DSS compared to Sham mice d. Representative images from marble burying conducted during the third DSS recovery week e. Both Sham+DSS and TBI + DSS buried significantly more marbles than their water-treated counterparts f. Data expressed as mean ± s.e.m (n = 15–21/group). b ** p = 0.01 vs Sham, *** p = 0.001 vs Naïve, ^^ p < 0.01 vs Naïve+DSS, ^^^ p < 0.0001 vs Naïve+DSS, + p < 0.05 vs TBI, ++++ p < 0.0001 vs Sham. c,d ** p = 0.01 vs Naïve/Sham, ^ p < 0.05 vs Naïve+DSS, ^^ p < 0.01 vs Naïve+DSS, ^^^ p < 0.0001 vs Naïve+DSS, + p < 0.05 vs Sham, ++ p < 0.01 vs Sham. e ^ p < 0.05 vs Naïve+DSS, ^^^ p < 0.001 vs Naïve+DSS, + p < 0.01 vs TBI, ++++ p < 0.0001 vs Sham