Fig. 1

Effect of neonatal HI on miR-210 level, TET2 protein abundance, and p65 acetylation level and its DNA binding at IL-1β promoter in the mouse brain. HI brain injury was induced in postnatal day 7 (P7) mouse pups, the ipsilateral cerebral hemisphere of the brain was collected at 3, 12, and 24 h after HI. MiR-210 levels were detected in the ipsilateral hemisphere in Sham and HI in both male (a) and female pups (b) using qRT-PCR. n = 4 pups/group. *p < 0.05 vs Sham. At 12 h after HI, c TET2, d acetyl-p65, and total p65 protein levels were detected by Western blot. Data are expressed as mean ± SEM. n = 6 pups/group. *p < 0.05 vs Sham. e Chromatin immunoprecipitation (ChIP)-PCR assay of acetyl-p65 at the promoter of IL-1β. The enrichment of IL-1β was detected by qPCR. Data are expressed as mean ± SEM. n = 4 pups/group. *p < 0.05 vs Sham