Fig. 3

TET2 interacting with HDAC3 regulated p65 acetylation at IL-1β promoter. a HI brain injury was induced in mouse pups, and the ipsilateral cerebral hemisphere of the brain was collected 12 h after HI. Co-immunoprecipitation was applied using a specific antibody against TET2. The specific binding of TET2, p65, and HDAC3 was detected by Western blot using specific primary antibodies. Either TET2 siRNA (100 pmol; si. Tet2) or scramble (si. Ctrl) was administered into the right hemisphere of mouse brain via i.c.v. injection, and 48 h later, b TET2 protein level was detected by Western blot. Data are expressed as mean ± SEM. n = 4 pups/group. *p < 0.05 vs si. Ctrl. c Chromatin immunoprecipitation (ChIP)-PCR assay of acetyl-p65 at the promoter of IL-1β. The enrichment of IL-1β was detected by qPCR. Data are expressed as mean ± SEM. n = 5–6 pups/group. *p < 0.05 vs Sham