Fig. 7

TET2 knockdown counteracted the effect of miR-210 inhibition on inflammatory response in neonatal HI brain injury and in BV2 microglia cell line in vitro. Either TET2 siRNA (100 pmol; si. Tet2) or scramble (si. Ctrl) was administered into the right hemisphere of both male and female mouse brain via i.c.v. injection, neonatal HI was induced 48 h after si. Tet2 injection. Four hours after HI, either miR-210-LNA (100 pmol) or scramble LNA (Neg. Ctrl) was administered into the ipsilateral hemisphere of mouse brain via i.c.v. a Brain infarct size was measured by TTC staining 48 h after HI. Data are expressed as mean ± SEM. n = 8–9 pups/group. *p < 0.05 vs Neg. Ctrl-treated si. Ctrl. ^#p < 0.05 vs miR-210-LNA-treated si. Ctrl. b The foot-fault test was performed 2 weeks after neonatal HI. Data are expressed as mean ± SEM. n = 9–12 pups/group in Neg. Ctrl-treated groups; n = 14–15 pups/group in LNA-treated groups. *p < 0.05 vs Neg. Ctrl-treated si. Ctrl. ^#p < 0.05 vs miR-210-LNA-treated si. Ctrl. c The ipsilateral cerebral hemisphere of the brain was collected 24 h after HI. The qRT-PCR was performed for IL-1β transcript. Data are expressed as mean ± SEM. n = 4–5 pups/group. d BV2 cells were transfected with miR-210 mimic, si. Tet2 or controls for 24 h. The qRT-PCR was performed for IL-1β transcript. Data are presented as the mean ± SEM. n = 3. e BV2 cells were stimulated with multiple doses of LPS for 6 h. The qRT-PCR was performed for IL-1β transcript. Data are presented as the mean ± SEM. n = 3. e BV2 cells were transfected with either miR-210-LNA or Neg. Ctrl in combination with si. Tet2 or si. Ctrl for 24 h followed by LPS stimulation (1 ng/ml or 500 ng/ml) for 6 h. The qRT-PCR was performed for IL-1β transcript. Data are presented as the mean ± SEM. n = 6 in groups with 1 ng/ml LPS; n = 3 in groups with 500 ng/ml LPS. *p < 0.05 vs Neg. Ctrl-treated si. Ctrl. ^#p < 0.05 vs miR-210-LNA-treated si. Ctrl. ns, not significant