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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Microglial deletion and inhibition alleviate behavior of post-traumatic stress disorder in mice

Fig. 1

Identification and characterization of immunocyte populations in the mouse brains by mass cytometry. a Schematic of mass cytometry procedure. In each group, the brains were separated from five mice, dissociated into single-cell suspensions, mixed as a single sample, barcoded, pooled, and stained with metal-tagged primary antibodies. The samples were analyzed by the CyTOF machine, and immunocyte populations were identified and characterized using visualization tool for statistical epistasis networks (viSNE) and heatmap analysis. b viSNE map displaying immune cells from control mice. Colors represent different cell populations clustered by Flow Self Organizing Map (FlowSOM). c Clustering and expression level of functional markers in immunocytes from the naïve brains. d Frequency distributions of immunocytes in the control and PTSD model brains. (FS foot-shocks, SCS single-cell solution)

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