Fig. 1

Transgene expression analysis in the brain. a hHSPB1 protein expression level was measured in whole-brain homogenates 24 h after ethanol treatment using Western blot. Mouse β-actin was used as an internal control. b Quantification of the results was performed using the open-source ImageJ software. Fold changes were correlated with the transgenic control group. Data are represented as mean ± SEM; n = 3 mice per group. c Relative expression of the transgenic hHSPB1 24 h after ethanol treatment was studied in the brain using RT-PCR. Fold changes were correlated with the transgenic control (nontreated) group. Values are presented as mean ± SEM; n = 8 mice per group. d hHSPB1 immunolabeling on sagittal brain sections of saline- and ethanol-treated transgenic mice 24 h after treatment. Scale bar: 500 μm (Ctx, Hip) and 100 μm (OCx and RSCx). Ctx cortex, Hip hippocampus, RSCx retrosplenial cortex, OCx occipital cortex. e Fluorescent double immunohistochemistry on sagittal brain sections showing cell type–specific expression of the transgene in the cortical region. Scale bar: 20 μm. Red: hHSPB1; green: IBA1/NEUN/GFAP; blue: DAPI