Fig. 5

Apoptotic cell death detection using TUNEL assay 24 h after EtOH treatment. a Programmed cell death was detected on sagittal brain sections of control and EtOH-treated hHSPB1-overexpressing and wild-type mice after 24 h of the respective treatment. Scale bar: 2000 μm. b Apoptotic cells were counted in the brain of transgenic and wild-type mice 24 h after EtOH treatment. Values are presented as mean ± SEM; n = 6 mice per group. Asterisk indicates wild-type EtOH group vs wild-type control group; plus sign indicates transgenic EtOH group vs transgenic control group. Statistical analysis: 2-way ANOVA followed by Tukey post hoc test. *^/+p < 0.05; **^/++p < 0.01; ***p < 0.001. B whole brain, Ctx cortex, Hip hippocampus, Th thalamus, Str striatum, Mb midbrain, Cb cerebellum