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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Neuroinflammatory processes are augmented in mice overexpressing human heat-shock protein B1 following ethanol-induced brain injury

Fig. 6

Characterization of the established primary cell cultures. Evaluation of the purity of primary a microglia, b astrocyte, and c neuronal cultures using fluorescent immunohistochemistry and detection of the presence of microglial (IBA1—green), astrocytic (GFAP—green/red), and neuronal (MAP2—red) markers. Cell nuclei were counted for cell-specific markers and percentages compared to the total cell number are given. Scale bar: 100 μm. Values are presented as mean ± SEM; n = 3–11. d Analysis of the expression pattern of the transgenic hHSPB1 protein in primary cell cultures using double immunofluorescent staining. Scale bar: 50 μm. Green: hHSPB1; red: IBA1/MAP2/GFAP; blue: DAPI

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Journal of Neuroinflammation

ISSN: 1742-2094