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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Neuroinflammatory processes are augmented in mice overexpressing human heat-shock protein B1 following ethanol-induced brain injury

Fig. 8

Effects of cytokine and EtOH treatment on intracellular hHSPB1, MAP2, and GFAP levels in transgenic cultures. a hHSPB1 and MAP2 double immunofluorescence staining in primary neuronal culture from hHSPB1-overexpressing mice 24 h after EtOH and cytokine treatment. Scale bar: 200 μm. Red: MAP2; green: hHSPB1; blue: DAPI. b Quantification of MAP2-positive area in transgenic primary neurons. n = 12. c Quantification of hHSPB1 fluorescent intensity in transgenic primary neurons. n = 12. d hHSPB1 and GFAP double immunofluorescence staining in primary astrocyte culture from hHSPB1-overexpressing mice 24 h after EtOH and cytokine treatment. Scale bar: 50 μm. Red: GFAP; green: hHSPB1; blue: DAPI. e Quantification of GFAP fluorescent intensity in transgenic primary astrocytes. n = 9–12. f Quantification of hHSPB1 fluorescent intensity in transgenic primary astrocytes. n = 10–11. Data are expressed as a percentage of untreated cells. Values presented are means ± SEM. crtl untreated control, EtOH ethanol treatment, cyt cytokine treatment. Statistical analysis: unpaired two-tailed t test. *p < 0.05; ***p < 0.001 compared to control

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