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Table 1 Primers and parameters of RT-qPCR

From: Crosstalk between stressed brain cells: direct and indirect effects of ischemia and aglycemia on microglia

RNA

Sequences forward/backward 5′-3′

Temperature (°C), step 1/2/3

Duration (s), step 1/2/3

iNOS

GCTTGTCTCTGGGTCCTCTG/

CTCACTGGGACAGCACAGA

95/59/72

15/15/45

CD206

AACAAGAATGGTGGGCAGTC/

CCTTTCAGTCCTTTGCAAGC

95/56/72

15/15/45

TNFα

CATCCGTTCTCTACCCAGCC/

AATTCTGAGCCCGGAGTTGG

95/56.6/72

15/15/45

IL-6

CCCAACTTCCAATGCTCTCCT/

AGCACACTAGGTTTGCCGAG

95/57.3/72

15/15/45

IL1-β

GACTTCACCATGGAACCCGT/

GGAGACTGCCCATTCTCGAC

95/56/72

15/15/45

IL-10

GAAAAATTGAACCACCCGGCA/

TTTCCAAGGAGTTGCTCCCG

95/56/72

15/15/45

RPL13a

TCTCCGAAAGCGGATGAACAC/ CAACACCTTGAGGCGTTCCA

 

15/15/45