Fig. 5
CypA secretion and endocytosis in cultured pericytes. a ELISA results of CypA concentrations in pericyte culture media after Hb stimulation. @: vs. sham P < 0.05. b ELISA results of CypA concentrations in pericyte culture media pretreated with CypA siRNA at 24 h after Hb stimulation. @: vs. vehicle P < 0.05, $: vs. Hb + Scr siRNA P < 0.05. c Representative FITC positive cells in cultured pericyte after exogenous CypA-FITC stimulation. d Flow cytometry results of FITC-positive cell percentage after treatment of endocytosis inhibitors and CypA-FITC on cultured pericyte. @: vs. DMSO P < 0.05. #: vs. CypA-FITC P < 0.05. e ELISA results of MMP9 concentrations in pericyte culture media after Hb stimulation. @: vs. vehicle P < 0.05, #: vs. Hb + vehicle P < 0.05. f Representative zymography bands and g quantitative analysis of MMP9 activities from ipsilateral hemisphere after SAH. #: vs. Hb P < 0.05, $: vs. Hb + Scr siRNA. h ELISA results of MMP9 concentrations in pericyte culture media after Hb stimulation. @: vs. vehicle P < 0.05, #: vs. Hb + vehicle P < 0.05, $: vs. Hb + Scr siRNA P < 0.05. i ELISA results of MMP9 concentrations in pericyte culture media after Hb stimulation. @: vs. vehicle P < 0.05, #: vs. Hb + vehicle P < 0.05. n = 6