Fig. 3

Changes in the mechanical threshold after antagonist administration. a Compared to the CYP + DMSO groups, ANA-12 (0.5 mg/kg), and ANA-12 (1.0 mg/kg) treated every other day after CYP injection reduced the decrease in the mechanical threshold and accelerated recovery significantly, while there was no difference between ANA-12 (0.1 mg/kg) and CYP + DMSO group. There was difference between ANA-12 (0.5 mg/kg) and ANA-12 (1.0 mg/kg) only at day 7, day 10, day 13. b Compared to the CYP+DMSO groups, ANA-12 (0.5 mg/kg), and K252a treated every other day after CYP injection reduced the decrease of the mechanical threshold and accelerated recovery significantly, and there was no difference between the effects of ANA-12 (0.5 mg/kg) and K252a. c ANA-12 treated next day after the third CYP injection 3 days continuously could reverse the mechanical threshold rapidly. d ANA-12 treated one day prior to CYP injection could prevent the onset of allodynia; moreover, there was no significant difference when compared with the control group. All data were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean ± SEM (n = 10 per group). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + DMSO group. &&p < 0.01, &&&p < 0.001 vs. the ANA-12 (0.5mg/kg) group