Fig. 4

Antagonized TrkB could restrain the activation of astrocytes and microglia and supress the p38/JNK pathway to alleviate the release of IL-1β and TNF-α in the SDH of CYP-induced cystitis. Western blots showed that the overexpression of a BDNF, b TrkB, c p-TrkB, d Iba1, e GFAP, f p-p38, g p-JNK, h TNF-α, and i IL-1β were downregulated in comparison to the CYP + DMSO group after ANA-12 treatment. All data were calculated as mean ± SEM (n = 5 per group). **p < 0.01, ***p < 0.001 vs. control group. ##p < 0.01, ###p < 0.001 vs. CYP + ANA-12 group