Fig. 4

HI affects microglial morphology and phagocytosis. A1, A2, A3 Microglia were classified upon their morphology to A1 amoeboid, A2 intermediate, or A3 ramified (arrows). B Proportions of Iba1+ microglia in the dorsolateral SVZ according to their morphology. Statistical analysis for ramified microglia. Mean with standard error of the mean (error bar). C 3D-reconstructed representative image highlighting the content of ball-and-chain microglial buds (arrows) that consist of pyknotic DAPI+ cells. D1, D2, D3, D4, D5 Representative images of a pyknotic BrdU+ cell engulfed by a microglial bud (arrow) with D1 DAPI, D2 BrdU, D3 Iba1 stainings, D4 the correspondent composite stack image, and D5 a 3D-reconstructed image of the whole microglia cell. E1, E2, E3, E4 Representative images of a pyknotic CC3+ cell engulfed by a microglial bud (arrow) with E1 DAPI, E2 CC3, E3 Iba1 stainings, and E4 the correspondent composite stack image. F Number of ball-and-chain buds per Iba1+ microglia in the dorsolateral SVZ and the cortex. The polka dot-filled bar area indicates the mean number of BrdU+ containing ball-and-chain buds. Individual data shown as dots, bars as mean with SD (error bar). Two-way ANOVA with Tukey post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001 (Additional file 1: Table S3). Scale bar for A, C, D, E, 10 μm