Fig. 4
From: MicroRNA-7, synergizes with RORα, negatively controls the pathology of brain tissue inflammation
RORα is a novel target of miR-7 in BTI model. WT mice and miR-7def mice were intraperitoneally injected with LPS (2.5 mg/kg of body weight). After 12 h, brain tissue was collected. The global gene expression was analyzed by cDNA chip array. a Heatmap and b scatter plot of gene expression. c The fold change and frequency. d Prediction of 16 target genes by using miRDB database (http://mirdb.org) and venn analysis. e The fold change of the potential target genes of miR-7 in the brain tissue was analyzed by real-time PCR assay and calculated (n = 3, one-way ANOVA). f, g The expression level of RORα was also determined by IF and h, i IHC assay (n = 3, one-way ANOVA). j Luciferase assay (n = 3, one-way ANOVA, **P < 0.01)