Table 1 The primer sequence used for real-time quantitative PCR
From: MicroRNA-7, synergizes with RORα, negatively controls the pathology of brain tissue inflammation
Gene | Primer sequence (5′-3′) |
|---|---|
TGF-β | F: CCCCATTCCTACTTCTCC |
R: ACGCACCTTTCTGGTTACAC | |
IL-6 | F: AGACAAAGCCAGAGTCCTTCAG |
R: GGTCTTGGTCCTTAGCCACTC | |
TNF-α | F: TGTCTACTGAACTTCGGGGTG |
R: CTGCTCCTCCACTTGGTGGTT | |
IL-1β | F: GAGCTTCAGGCAGGCAGTAT |
R: TTGTTCATCTCGGAGCCTGTA | |
TROVE2 | F: GAAGTGTGTCGCATTCCGACC |
R: GGACAGTCGGAGGAGATCTTT | |
RORα | F: GGAGACAAATCGTCAGGAATC |
R: ACCAAACTTGACAGCATCTCG | |
Col4a3bp | F: TTGAAGCTGCTCTTGACAGAC |
R: TCTATGCGTCCCGACAGAAGA | |
Gpr158 | F: AATTAGAAGCAGCCCAATGG |
R: TTTCACGAACAGCACAAAGAA | |
Zfp212 | F: ACCAAGTCACCCACCATCTCT |
R: GAGAACCTGCTTCGAAACAA | |
Socs4 | F: CCCCAGTGCCTGTATGTTCTT |
R: CTGCTGCTCTGGCACATCAAT | |
C2cd2 | F: TCAGGCCTTAGCCATGTGT |
R: CGTGGGGACTTGAGTTTCA | |
Syt4 | F: GAGAAGCTGGGGACACTCTT |
R: ACGGGTCAGAGGTCATGGATT | |
Spock3 | F: CAGTCTGTGGTTCTGATGGGC |
R: GACTTATCGGAGGGACATGG | |
Gpr22 | F: GCAAAACACCAACTGCTCCAA |
R: ACTGCATGTTGATTTCCAGAA | |
Basp1 | F: CTCTTTGACGGCCACGCTTTG |
R: CTGAGCAAGAAGAAGAAGGGCT | |
Tmed5 | F: TCGATCTCCAACGATGCCTT |
R: TCACACCCTCTTTGGACAGTG | |
Ptprj | F: TGCCATTTGCATTGCTCCAG |
R: CTTAAGCCCAGGCACTTCGT | |
Zfp606 | F: TCATGGACCAGTCTTGGGGG |
R: ATCTCACCTCGACTTGGGCTA | |
Aqp11 | F: TGCAGGAATCCCATCCACAC |
R: CCCTCCTGCATAGGCCAAAA | |
1110059G10Rik | F: TCTTCAGCTGTTAGGTCTCCC |
R: GAGCAAGCGGAACCAAGTGT | |
Gtdc1 | F: CACCCTTCTGTGTGGAGCTGA |
R: TTACGGGGTAAGTAGCCCCA | |
GAPDH | F: GAAGGTCGGAGTCAACGGATT |
R: ATGGGTGGAATCATATTGGAA | |
miR-7 | F: CGGCGGTGGAAGACTAGTGATT |
U6 | F: AGAGAAGATTAGCATGGCCCCTG |
Common reverse | R: ATCCAGTGCAGGGTCCGAGG |