Fig. 3
From: ADAMTS13 ameliorates inflammatory responses in experimental autoimmune encephalomyelitis
Effect of preventive ADAMTS13 treatment on leukocyte infiltration into the spinal cords of EAE mice. The whole spinal cords from ADAMTS13- or vehicle-treated EAE mice at 22 dpi were isolated and measured by flow cytometry (a, b). Leukocyte populations were gated as follows: T lymphocytes (CD45hi CD11b− CD3+), microglia (CD45mild CD11b+), neutrophils (CD45hi CD11b+ Ly6Cint Ly6Ghi), and monocytes (CD45hi CD11b+ Ly6Chi Ly6G−). a Representative plots of leukocyte subsets (pre-gated on CD45hi leukocytes) in the spinal cords from EAE mice treated with vehicle or ADAMTS13. b The numbers and frequencies of leukocyte subsets compared to CD45hi leukocytes in the spinal cords of EAE mice (n = 6 each group). c Representative immunofluorescence images of spinal sections from EAE mice at 22 dpi are shown. Tissue sections were stained with antibodies against CD3 (upper; green, T lymphocytes), Iba1 (middle; red, macrophages/microglia), and Ly6G (bottom; green, neutrophils). DAPI was used to stain the nucleus (blue staining). Bars, 20 μm. d Quantitative analysis of immunofluorescence images is shown (n = 4 each group). Values are expressed as the mean ± SEM, and Student’s t test was used to compare differences between groups. *P < 0.05; ***P < 0.001; ns no significance