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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: ADAMTS13 ameliorates inflammatory responses in experimental autoimmune encephalomyelitis

Fig. 7

Therapeutic treatment with ADAMTS13 reduces neurological deficits in established EAE mice. Fifty micrograms of ADAMTS13 or vehicle was injected into the lateral ventricle of EAE mice every day from 15 dpi until 29 dpi, and clinical scores were recorded daily. a The daily mean clinical score is shown, and the mean clinical scores at 29 dpi were compared between vehicle and ADAMTS13 group (***P < 0.001). b Cumulative clinical score was summed by adding daily clinical scores from 15 dpi to 29 dpi. c Maximum clinical scores of EAE mice were compared between groups. Statistical significance was determined by the Mann-Whitney U test for clinical scores. d Lumbar spinal cord sections of EAE mice at 30 dpi were subjected to LFB (upper panels) and HE (lower panels) staining for assessment of demyelination and inflammation, respectively. The arrowhead shows demyelinating lesions, and the arrow shows the area of inflammation. Scale bars represent 100 μm and 200 μm. The percent of white matter demyelination and the level of inflammatory cell infiltration of the spinal cord were compared between ADAMTS13- and vehicle-treated EAE mice by Student’s t test (n = 4). e Tissue sections of spinal sections from EAE mice at 30 dpi were stained with antibodies against VWF (red) and DAPI (blue, nucleus). Bars, 20 μm. f Quantitative RT-PCR analysis of IL-1β, CXCL1, and CCL2 mRNA levels in the spinal cords of ADAMTS13- and vehicle-treated EAE mice at 30 dpi. Data represent fold changes compared to the spinal cords of naïve mice (n = 6). Values are expressed as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001

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