Fig. 2

High-salt diet does not affect neuroinflammation in MSA mice. a Heat map comparing the log2 fold change in cytokine and chemokine expression in different brain regions of MSA mice fed with/without high-salt diet and control mice fed with high-salt, as referred to control mice fed with a normal diet. b Protein levels of CCL3, CCL4 and CCL5 chemokines in the midbrain. Protein levels are expressed in pg of protein of interest per mg of total proteins. c Relative gene expression of T-cell markers Cd4 and Cd8 in the midbrain. The data is expressed in fold change relative to WT mice fed with a normal diet. White bars: WT mice; gray bars: WT mice fed with HSD (TG + HSD); black bars: TG mice; red bars: TG mice fed with HSD (TG + HSD). Error bars indicate SEM. Two-way ANOVA: *p < 0.05, **p < 0.01, ****p < 0.0001 (Bonferroni’s test)