Fig. 4
High-salt diet does not interfere with α-syn accumulation in MSA mice. a Representative images of SN sections stained against phosphorylated α-syn. Red arrows indicate individual GCI. Scale bar, 25 μm. b GCI density in SNc, CBWM, and motor cortex (M2) of PLP-hαSyn mice determined by stereological counting of brain sections stained against phosphorylated α-syn and expressed in GCI/mm2. c Representative images of DOT BLOT analysis for soluble human α-synuclein levels in the midbrain. d Quantification of soluble human α-synuclein levels in different brain areas. The data is shown in arbitrary units (a.u.). Error bars indicate SEM. Two-way ANOVA): ***p < 0.001, ****p < 0.0001 (Bonferroni’s test)