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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense

Fig. 1

GPER activation attenuates neuroinflammation in the hippocampal CA1 region following GCI and enhances neuronal survival. a Representative images of double-immunofluorescent staining for GPER (green) and Iba1 (red) in indicated groups (yellow), indicating co-localization of GPER with Iba1, and DAPI (blue) counter stains nuclei. Quantitative analysis of Iba1 intensity (b) and co-localization of GPER with Iba1 intensity (ratio to sham) of hippocampal CA1 region (c). d Representative images of double-immunofluorescent staining for NeuN (red) and GPER (green) in indicated groups, which shows that G1 administration enhanced GPER immunoreactive levels in hippocampal neurons. e Representative photographs of NeuN staining (green) and TUNEL (red) in the indicated groups (reperfusion at 14 days). Quantification was performed by counting the number of NeuN-positive neurons (f) or TUNEL-positive (g) per 250 μm length in the medial CA1 pyramidal cell layer. Magnifications are zooms of the boxed areas. Scale bar, 50 μm. n = 7–8, *P < 0.05 vs. IR group, #P < 0.05 vs. G1-treated group. IR: ischemia reperfusion

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Journal of Neuroinflammation

ISSN: 1742-2094